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. 2020 Aug 27;11:4159. doi: 10.1038/s41467-020-17969-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a In situ hybridization for Wnt2 mRNA during tracheoesophageal segregation. Arrowheads indicate Wnt2 expression in the ventrolateral mesoderm at E9.5 and E10.5. n = 2/2 embryos. b Transverse sections of Shh^Cre, Wls^flox/flox mouse embryos and littermate controls at E10.5. Left panels show sections stained with Sox2 (green), Tbx4 (magenta), and DAPI (blue). Right panels show sections stained for Nkx2.1 (magenta) and DAPI (blue). n = 3/3 embryos per genotype. c In situ hybridization for Wnt2 mRNA in Shh^Cre, Wls^flox/flox mouse embryos and littermate controls at E9.5. Arrowheads indicate Wnt2 expression in the ventrolateral mesoderm. n = 2/2 embryos. d Refined model of tracheoesophageal segregation and tracheal mesodermal differentiation. e In situ hybridization for Wnt7b mRNA in mouse embryo at E10.5. Arrowhead indicates Wnt7b⁺ cells. n = 2/2 embryos. Eso Esophagus, Lu Lung, Tr Trachea. Scale bar; 40 μm (a–c), 50 μm (e).