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. 2020 Aug 27;11:4289. doi: 10.1038/s41467-020-18039-x

Fig. 5. Old human organ donors had increased systemic levels of cf-mt-DNA activating DCs.

Fig. 5

a Cf-mt-DNA was quantified in the plasma of young (<35 years) and old (>55 years) organ donors by RT-PCR using TAQMAN primers for mt-Co3 (p = 0.0013) and mt-nd6 (p = 0.0007), Scatter plots display mean ± SEM. b Dendritic cells were differentiated from isolated PBMC, stimulated with human mt-DNA (10 μg/ml), and costimulatory molecule expression was analyzed using flow cytometry (p = 0.0022). Column plots display mean ± SD (n = 6 biologically independent samples); results are representative of at least three independent experiments. Statistical significance was determined by using two-tailed Mann–Whitney-test. Asterisks indicate p values *p ≤ 0.05, **p ≤ 0.01, and ***p ≤ 0.001, only significant values are shown. Source data are provided as a Source Data file.