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. 2020 Aug 27;11(8):710. doi: 10.1038/s41419-020-02900-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a, b The mRNA and protein expression levels of PGAM1 in bronchial epithelial cell 16HBE and NSCLC cell lines (A549, H1299, NCI-H226 and SK-MES-1) were analyzed by RT-qPCR (up panel) and western blot (lower panel). c NCI-H226 and SK-MES-1 cells were untreated (Blank), transfected with negative control (shCtrl), or transfected with shRNAs targeting PGAM1 (sh-PGAM1-1/2/3). The expression of PGAM1 protein was analyzed by western blot 48 h later. The representative result of three independent experiments was shown. Cell proliferation capability of NCI-H226 and SK-MES-1 cells transfected with shCtrl or shPGAM1-1/2 was determined by CCK-8 assay (d), EdU assay (e) and colony formation assay (f). Transwell experiment (g) and wounding healing assay (h) was performed to analyze the cell invasion and migration capability of NCI-H226 and SK-MES-1 cells transfected with shCtrl or shPGAM1-1/2. The results are presented as the mean ± SD. *p < 0.05, **p < 0.01 by Mann–Whitney U-test.