Fig. 5.

Cell viability after drug addition. (A) Laser confocal photographs of cells cultured on a chip for 24 h before drug addition. Scale bar = 500 μm. (B) Laser confocal photograph of cells 48 h after drug addition. The scale bar in the first row is 500 μm, the second row is 200 μm. (C) Statistics on the number of cells in different culture chambers after three days of cultivation. (D) The IC50 curve obtained by the conventional method was measured; that is, the cells were cultured on a well plate, and the OD value was determined by adding the CCK-8 reagent.