Figure 5.

Quizartinib- and dexamethasone-induced synergistic cell death is mediated through GR. A representative immunoblot (A) and quantification of replicate experiments (B) (denoted by the individual dots) using densitometry showing GR is increased following 48-hour quizartinib (100 nM) treatment in MV4-11 cells. (C) Immunoblot showing GR-knockout (pooled single clones) in MV4-11 cells. (D) FACS quantification of dead cells (Annexin-V positive) in GR-knockout and wild-type MV4-11 cells treated with the indicated drugs (100 nM quizartinib, 50 µM dexamethasone) for 48 hours. KO, knockout.