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. Author manuscript; available in PMC: 2020 Aug 28.
Published in final edited form as: Hepatology. 2019 Jun 24;70(6):2123–2141. doi: 10.1002/hep.30766

FIG. 1.

FIG. 1.

ALD in mice and humans is associated with impaired autophagic flux. (A,B) Total liver proteins were extracted from pair-fed (PF), chronic alcohol diet (EtOH), and acute-on-chronic alcohol (EtOH) fed mice (n = 8–10/group) and analyzed by western blotting using β-actin as a loading control. Protein levels of Beclinl, Atg7, LC3-I, LC3-II, and p62 in chronic alcohol (A) and in acute-on-chronic alcohol-fed (B) mice. Western blotting analysis for LC3-I, LC3-II, and p62 from livers of control subjects and ALD patients (C). mTOR protein levels in mouse livers in chronic alcohol- and in acute-on-chronic alcohol-fed mice and in ALD patients (D). The densitometry analysis is shown as bar diagrams. Human liver sample data are representative of 3 control donors and 6–8 patients with cirrhosis and superimposed AH. *P < 0.05; **P < 0.01. Abbreviations: Con., control; ctrl., control.