FIG. 4.

Chronic alcohol-induced increase in miR-155 regulates autophagy at the autophagosome formation stage by targeting lysosome-associated membrane proteins. Total liver protein was extracted from pair-fed (PF) or chronic alcohol-fed (EtOH) WT and miR-155 KO mice (n = 8–10) and analyzed for LAMP2 and LAMP1 (A,B) by western blotting using β-actin as a loading control. Representative IHC images of LAMP2 from WT and miR-155 KO mice in livers (C). LAMP1 and LAMP2 IHC images from livers of healthy subjects and from patients with ALD (n = 64) (D). Macrophages (RAW 264.7) or hepatocytes (Hepa1–6) were untreated or transfected with either a miR-155 mimic or a control mimic and the LAMP2 protein levels determined by western blotting (E). *,#P < 0.05. Abbreviations: Con., control; ox, overexpression.