Fig. 1. Schematic of synthetic biochemistry pathway for conversion of glucose to isobutanol.

Enzymes used in this pathway are described in Table 1. Enzymes in red are from hyperthermophilic organisms, enzymes in blue are mesophilic and enzymes highlighted purple are designed specifically for this study. G3P glyceraldehyde-3-phosphate; 3PG 3-phosphoglycerate; 1,3-BPG 1,3-bisphosphoglycerate; Pyr pyruvate. The logic of the system including the rheostat are described in Opgenorth et al.²⁷.