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. 2020 Aug 27;11:4292. doi: 10.1038/s41467-020-18124-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Isobutanol and NADPH titers over time for 200 µL reactions. b Effect on isobutanol titers by the addition of 10 mM NADPH or NADP⁺ at 25 hours, the time at which the reaction achieves approximately maximum titers. The inset is a magnification of the final portion of the graph. NADPH addition raises isobutanol titer, while NADP⁺ reduces isobutanol titer. c The effect of the addition of 100 mM isobutanol at 25 hours on the NADPH levels at 25.5 h. The addition of isobutanol increases the NADPH concentration. d Isobutanol titer over time with addition of an organic overlay. Error bars in each panel represent the standard deviation of technical triplicates. Open circles in panel c show the individual data points. Source data are provided as a Source Data file.