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. 2020 Aug 27;11:4292. doi: 10.1038/s41467-020-18124-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Bioreactor set up for continuous product extraction. Phenetole in continuously added from a reservoir and simultaneously extracted at the same rate. The extracted layer is collected in a separate container. b Isobutanol titer over time using the continuous extraction system. c Residual activity of enzymes after 70 hours of incubation. d Isobutanol titer over time with the addition of a second bolus of KivD-S and BsAlsS-P at 33 h, the enzymes that become inactivated after 70 h. The ATP (green) and NADPH (blue) concentrations in the reaction are also shown as a function of time. Error bars in all panels are the standard deviation of technical triplicates. Open circles in panel c show the data points. Source data underlying Fig. 5b–d are provided as a Source data file.