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. 2020 Aug 19;2020:8857344. doi: 10.1155/2020/8857344

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Copyright © 2020 Li Chen et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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The technical flow chart of inducible inhibition or activation-specific genes in CRISPRi or CRISPRa human stromal/stem cells (hMSCs). Human bone marrow stromal/stem cell line (hMSC-TERT) stable transfected with dCas9 TRE-KRAB or dCas9TRE-VP64 to establish the inducible CRISPRi or CRISPRa cell lines. In these cells, with simply transfecting the small molecular guide RNAs (gRNAs) that target different specific genes, it can easily inhibit or activate specific gene expressions with or without doxycycline (Dox) in cell culturing mediums or differentiation induction medium.