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Sequences of primers designed for RT-qPCR.
| Gene | Forward sequences | Reverse sequences | GenBank accession # of mRNAs |
|---|---|---|---|
| GPX1 | AGTCCACCGTGTATGCCTTCTCC | TCTCTTCATTCTTGCCATTCTCCTG | NM_030826 |
| GPX4 | GGAGGCAGGAGCCAGGAAGTAA | AGCCGTTCTTATCAATGAGAAACTTGG | NM_001039849 |
| β-Actin | CGTTGACATCCGTAAAGACCTC | TAGGAGCCAGGGCAGTAATCT | NM_031144 |
GPX1: glutathione peroxidase 1; GPX4: glutathione peroxidase 4. β-Actin was used as an internal control to normalize target gene levels.
