Table 1. Bacterial strain and plasmid used in this study.
| Strain or plasmid | Relevant characteristics | Reference or origin |
|---|---|---|
| Strains | ||
| Nocardioides sp. PD653 | HCB+ | 2 |
| E. coli DH5α | F−, l−, f80dlacZDM15, D(lacZYA-argF)U169, deoR, recA1, endA1, hsdR17(rk−, mk+), phoA, supE44, thi-1, gyrA96, relA1 | TOYOBO |
| Rhodococcus erythropolis L88 | A lysozyme sensitive mutant derived from Rhodococcus erythropolis JCM3201 | Hokkaido system science Co., Ltd. |
| Plasmids | ||
| pNitRC1 | E. coli-R. erythropolis shuttle vector, Apr for DH5α, Cmr for L88, Pnit, rep (pRE8424) | Hokkaido system science Co., Ltd. |
| pCpiRC1 | E. coli-R. erythropolis shuttle vector, Apr for DH5α, Cmr for L88, Pcpi, rep (pRE8424) | Hokkaido system science Co., Ltd. |
| pCpiRC1(hcbA1)C-His | pCpiRC1 with PCR-amplified DNA fragment of hcbA1 | This study |
| pCpiRC1(hcbA2)C-His | pCpiRC1 with PCR-amplified DNA fragment of hcbA2 | This study |
| pNitRC1(hcbA3)C-His | pNitRC1 with PCR-amplified DNA fragment of hcbA3 | This study |
HCB+, ability of degrade HCB; Apr, ampicillin resistant; Cmr, chloramphenicol resistant.