MT-KO and 129S1 mice were exposed to IH for indicated times. MT-TG mice and FVB mice were exposed to IH for 4 weeks. The expression of p-PI3K and t-PI3K (A and E), p-Akt and t-Akt (B and F), p-GSK-3β and t-GSK-3β (C and G), Fyn (D and H) in heart was detected by Western blot assay and the ratio of p-PI3K and t-PI3K, p-Akt and t-Akt, p-GSK-3β and t-GSK-3β was presented. Data are presented as mean ± SD (n=5). *, p<0.05 vs WT/C; & p<0.05 vs WT/H.