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The selectivity filter of closed-gate wt KcsA soaked in K+/Ba2+ solution. The concentration of K+ increases from left to right; the concentration of Ba2+ is fixed at 5 mM. The Fo − Fc density (green mesh), countoured at 8 σ, shows the positions of K+ along the selectivity filter. No anomalous signal is observed for Ba2+ for the closed-gate KcsA soak structures.
