Table 2.
Data collection and refinement statistics of closed-gate Kcsa
| KcsA closed-gate soaked in 5 mM BaCl2 | KcsA closed-gate | ||||
|---|---|---|---|---|---|
| 0 mM KCl | 1 mM KCl | 5 mM KCl | 10 mM KCl | Incubated in Ba2+/Na+ | |
| Wavelength (Å) | 0.9791 | 0.9791 | 0.9791 | 0.9791 | 1.033 |
| Source | APS 24-ID-C | APS 24-ID-C | APS 24-ID-C | APS 24-ID-C | APS 23-ID-D |
| Resolution (Å) | 51.0–2.4 | 54.8–2.6 | 68.0–2.6 | 67.9–2.3 | 68.6–2.5 |
| Space group | I4 | I4 | I4 | I4 | I4 |
| Cell parameters | |||||
| (Å) | 155.0, 155.0, 75.3 | 155.1, 155.1, 75.3 | 155.8, 155.8, 75.5 | 154.2, 154.2, 75.7 | 156.3, 156.3, 76.3 |
| (°) | 90.0, 90.0, 90.0 | 90.0, 90.0, 90.0 | 90.0, 90.0, 90.0 | 90.0, 90.0, 90.0 | 90.0, 90.0, 90.0 |
| Total reflections | 243,853 | 189,833 | 252,932 | 399,672 | 306,876 |
| Unique reflections | 35,112 | 27,029 | 28,030 | 37,609 | 32,029 |
| Multiplicity | 7.0 (7.0) | 7.0 (7.0) | 9.0 (8.9) | 10.6 (9.1) | 9.6 (9.9) |
| Completeness (%) | 99.8 (99.9) | 97.2 (97.9) | 99.9 (99.9) | 98.6 (91.8) | 99.8 (99.8) |
| Mean I/sigma(I) | 7.6 (1.0) | 6.1 (0.9) | 5.9 (1.0) | 12.0 (1.1) | 7.1 (1.7) |
| R-merge | 0.18 (2.66) | 0.21 (2.50) | 1.03 (7.97) | 0.14 (2.42) | 0.23 (1.9) |
| R-pim | 0.11 (1.14) | 0.12 (1.04) | 0.38 (1.37) | 0.07 (0.81) | 0.08 (0.65) |
| CC1/2 | 0.99 (0.12) | 0.99 (0.13) | 0.84 (0.17) | 0.92 (0.29) | 0.99 (0.55) |
| Reflections used in refinement | 35,091 | 27,022 | 28,019 | 37,604 | 32,029 |
| R-work/R-free | 0.22/0.24 | 0.20/0.24 | 0.19/0.23 | 0.20/0.23 | 0.19/0.21 |
| RMS bond length (Å)/angle (°) | 0.01/2.2 | 0.01/1.3 | 0.01/1.3 | 0.01/1.2 | 0.01/1.3 |
| Ramachandran favored (%) | 96 | 96 | 96.2 | 95.6 | 95.4 |
| Allowed (%) | 3.9 | 3.7 | 3.7 | 4.1 | 4.3 |
| Outliers (%) | 0 | 0.1 | 0 | 0.1 | 0.1 |
Values in parentheses are for highest-resolution shell.