Fig. 2.

Age-dependent changes in OPC daughter cells in hippocampus of 3xTg-AD and age-matched non-Tg controls. (A) Confocal images illustrating OPC duplets (arrows), in the CA1 area of the hippocampus of 3xTg-AD mice immunolabelled for NG2 (green) and counterstained with the nuclear marker Hoechst blue (blue). Maximum intensity z-stack projection (Ai), together with single z-section (Aii) and orthogonal section through the x-x plane (Aiii), showing juxtaposed duplets of recently divided OPC daughter cells. Scale bars = 20 μm in Ai and 10 μm in Aii, Aiii. (B–E) Quantification of sister cells per constant field of view (FOV) in the hippocampus overall (B), the dentate gyrus (DG) (C), CA1 (D) and CA3 (E); data are mean ± SEM from 3 sections per animal, n = 3 animals. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, unpaired t-tests. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)