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. Author manuscript; available in PMC: 2020 Aug 28.
Published in final edited form as: Adv Med Sci. 2019 Dec 13;65(1):18–29. doi: 10.1016/j.advms.2019.10.002

Fig. 3. The multi-step protocol used to provide a multianalyte gene expression assay result for GEP-NETs.

Fig. 3.

A 2-step protocol (mRNA isolation and cDNA synthesis) is undertaken prior to quantitative PCR gene expression. Normalized 51-marker signature is interrogated using mathematical algorithms to provide a score that is scaled 0–100% (the NETest score). The NETest delineates in a specific patient whether the tumor falls into a category of low (< 40%), moderate (40–79%) and high (≥ 80%) risk for disease activity. HRS, hours; qPCR, quantitative PCR.