Table 3.

Summary of statistical trends of FLIM-derived features in Moderate and High-Grade Dysplasia/SCC vs. Normal. TRFS:Time-Resolved Fluorescence Spectroscopy; FLIM:Fluorescence Lifetime Imaging; MPM-FLIM:Multiphoton FLIM; AFS:Autofluorescence Spectroscopy; CFM:Confocal Fluorescence Microscopy; SLS:Synchronous Luminescence Spectroscopy

Emission Channel	Associated Fluorophore	FLIM-Derived Autofluorescence Features	Moderate and High-Grade Dysplasia / SCC vs. Normal
			Observed Trend	Previous Studies [Ref.]
				↓ In Vivo AFS: Hamsters [33]
		I390	↓	↓ Ex Vivo CFM: Patients [20]
		I390,n	↓	↓ Ex Vivo SLS: Patients [31]
				↓ In Vivo FLIM: Hamsters [32]
390 ± 20 nm	Collagen			↓ In Vivo TRFS: Patients [25]
		τa,v,g,390	↓	Not Reported
		αfast,390	No Change	Not Reported
		τfast,390	↓	Not Reported
		τslow,390	↓	Not Reported
				↓ In Vivo FLIM: Patients [2]
		I452	↓	↓ In Vivo AFS: Hamsters [33]
		I452,n	↓	↓ Ex Vivo SLS: Patients [31]
452 ± 22.5 nm	NADH	τa,v,g,452	↓	↓ In Vivo TRFS: Patients [25]
				↓ In Vivo FLIM: Patients [2]
				↓ In Vivo FLIM: Hamsters [32]
				↓ Ex Vivo FLIM: Patients [24]
				↓ In Vivo TRFS: Patients [25]
		αfast,452	↑	Not Reported
		τfast,452	↓	↓ In Vivo MPM-FLIM: Hamsters [18]
				↓ Ex Vivo TRFS: Patients [23]
		τslow,452	No Change	↓ In Vivo MPM-FLIM: Hamsters [18]
				↑ In Vivo AFS: Hamsters [33]
		I500	↓	↑ Ex Vivo CFM: Patients [20]
		I500,n	↑	↑ Ex Vivo SLS: Patients [31]
				↑ In Vivo TRFS: Patients [25]
> 500 nm	FAD	τavg,500	↑	↓ Ex Vivo FLIM: Patients [24]
		αfast,500	↑	↓ In Vivo MPM-FLIM: Hamsters [18]
		τfast,500	No Change	↑ In Vivo MPM-FLIM: Hamsters [18]
		τslow,500	↑	Not Reported
452 / 500 nm	Redox-Ratio = NADH / FAD	I452/I500	↓	↓ In Vivo AFS: Hamsters [33]
				↓ Ex Vivo FLIM: Patients [24]