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ICAII pretreatment upregulates the expression levels of p-PI3K and p-AKT following IR injury, while LY294002 abolishes this beneficial effect. Protein expression levels of (A) p-PI3K, total PI3K and p-PI3K/PI3K ratio and (B) p-AKT, total AKT and the p-AKT/AKT ratio were determined using western blotting. n=5. *P<0.05 vs. SO group; $P<0.05 vs. IR group; #P<0.05 vs. ICAII 20 + IR group. IR, ischemia-reperfusion; ICAII 20, 20 mg/kg icariside II; SO, sham-operated; p-, phosphorylated.
