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. 2020 Aug 5;22(4):3233–3244. doi: 10.3892/mmr.2020.11419

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Copyright: © Tang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — Effect of trehalose and sucrose during vacuum-drying of siRNA lipoplexes on the suppression of luciferase expression in MCF-7-Luc cells after reverse transfection with siRNA lipoplexes. LP-DOTAP, LP-DDAB and LP-TC-1-12 lipoplexes with 50 pmol Luc siRNA or Cont siRNA were diluted in 125 µl of 10, 25, 50, 100, 150, or 280 mM (0.43, 1.1, 2.1, 4.3, 6.4 and 12.0 mg, respectively) (A) trehalose or (B) sucrose and then transferred to 12-well plates, followed by vacuum-drying. Data are presented as the mean ± SD (n=3). *P<0.05 and **P<0.01 vs. Cont siRNA. siRNA, small interfering RNA; Luc, luciferase; Cont, control; LP-DOTAP, DOTAP liposome; LP-DDAB, DDAB liposome; LP-TC-1-12, TC-1-12 liposome.