Figure 8.

Effect of trisaccharide during the freeze-drying of siRNA lipoplexes on the suppression of luciferase expression in MCF-7-Luc cells after reverse transfection. (A) LP-DOTAP and (B) LP-DDAB lipoplexes with 50 pmol Luc siRNA or Cont siRNA were diluted in 125 µl of 10, 25, 50, 100, or 150 mM raffinose or melezitose solution and then transferred to 12-well plates, followed by freeze-drying. MCF-7-Luc cells suspended in culture medium (1 ml) were added to the well, and luciferase assays were performed after incubation for 48 h at 37°C. Data are presented as the mean ± SD (n=3). *P<0.05 and **P<0.01 vs. Cont siRNA. siRNA, small interfering RNA; Luc, luciferase; Cont, control; LP-DOTAP, DOTAP liposome; LP-DDAB, DDAB liposome.