Table II.
Particle size and ζ-potential of siRNA lipoplexes after the rehydration of freeze-dried lipoplexes.
| A, LP-DOTAP lipoplexesa | |||
|---|---|---|---|
| Saccharide, 25 mM | Sizeb, nm | PDI | ζ-potentialb, mV |
| Glucose | 322.3±5.5 | 0.27±0.01 | 45.1±2.2 |
| Fructose | 321.3±13.9 | 0.28±0.02 | 47.6±1.0 |
| Galactose | 231.6±7.3 | 0.29±0.01 | 40.4±0.7 |
| Mannose | 312.0±44.3 | 0.22±0.05 | 43.0±0.7 |
| Maltose | 185.0±3.9 | 0.25±0.01 | 50.1±0.3 |
| Lactose | 182.4±6.5 | 0.26±0.01 | 37.2±0.7 |
| Lactulose | 195.4±2.5 | 0.26±0.02 | 41.0±0.8 |
| Cellobiose | 184.1±1.6 | 0.25±0.01 | 44.6±1.1 |
| Raffinose | 183.2±2.7 | 0.25±0.01 | 36.3±0.4 |
| Melezitose | 206.0±11.1 | 0.26±0.01 | 27.5±1.3 |
| B, LP-DDAB lipoplexesa | |||
| Saccharide, 25 mM | Sizeb, nm | PDI | ζ-potentialb, mV |
| Glucose | 459.3±56.2 | 0.21±0.02 | 46.6±0.9 |
| Fructose | 1,041.8±266.1 | 0.42±0.09 | 45.7±1.4 |
| Galactose | 648.0±21.9 | 0.29±0.01 | 47.6±1.6 |
| Mannose | 323.9±27.7 | 0.15±0.01 | 53.9±2.7 |
| Maltose | 215.9±23.4 | 0.19±0.06 | 45.2±1.3 |
| Lactose | 162.3±3.0 | 0.18±0.00 | 36.7±2.8 |
| Lactulose | 217.0±13.8 | 0.23±0.01 | 38.8±0.4 |
| Cellobiose | 173.3±3.8 | 0.22±0.02 | 44.6±1.2 |
| Raffinose | 174.7±1.3 | 0.22±0.03 | 46.9±2.2 |
| Melezitose | 247.8±34.4 | 0.20±0.06 | 36.4±1.6 |
| C, LP-DOTAP lipoplexesa | |||
| Saccharide, 100 mM | Sizeb, nm | PDI | ζ-potentialb, mV |
| Glucose | 173.5±3.6 | 0.24±0.01 | 47.8±3.5 |
| Fructose | 219.6±0.6 | 0.24±0.02 | 46.5±0.7 |
| Galactose | 187.6±4.9 | 0.24±0.01 | 50.5±1.1 |
| Mannose | 219.6±0.6 | 0.24±0.02 | 46.5±0.7 |
| Maltose | 182.2±7.7 | 0.19±0.01 | 30.6±0.6 |
| Lactose | 187.3±1.3 | 0.14±0.01 | 30.9±0.6 |
| Lactulose | 188.8±6.4 | 0.14±0.01 | 46.3±0.7 |
| Cellobiose | 169.9±1.9 | 0.21±0.02 | 42.8±0.7 |
| Raffinose | 207.7±8.5 | 0.24±0.00 | 40.8±0.8 |
| Melezitose | 185.0±4.7 | 0.18±0.01 | 37.2±0.9 |
| D, LP-DDAB lipoplexesa | |||
| Saccharide, 100 mM | Sizeb, nm | PDI | ζ-potentialb, mV |
| Glucose | 171.9±4.1 | 0.23±0.01 | 43.8±1.9 |
| Fructose | 232.2±30.4 | 0.22±0.09 | 40.9±1.6 |
| Galactose | 235.6±38.3 | 0.20±0.05 | 43.3±2.3 |
| Mannose | 193.0±8.6 | 0.25±0.04 | 36.1±1.6 |
| Maltose | 193.1±0.8 | 0.22±0.00 | 43.5±2.2 |
| Lactose | 188.9±2.7 | 0.20±0.01 | 35.8±0.3 |
| Lactulose | 196.7±8.5 | 0.23±0.04 | 44.8±1.3 |
| Cellobiose | 179.8±3.2 | 0.24±0.00 | 40.1±0.4 |
| Raffinose | 162.5±0.8 | 0.18±0.01 | 44.1±1.5 |
| Melezitose | 197.7±1.7 | 0.21±0.01 | 15.3±1.0 |
a
Charge ratio (+:-) of cationic lipid to siRNA phosphate of 4:1.
b
In water. Data are presented as the mean ± SD (n=3). PDI, polydispersity index; siRNA, small interfering RNA.