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. 2020 Aug 11;22(4):3245–3254. doi: 10.3892/mmr.2020.11427

Figure 6.

Figure 6.

Downregulation of miR-143-3p reverses the suppressive effects of si-TMPO-AS1 on cell proliferation, migration and invasion in CRC cells. (A) Expression of miR-143-3p was detected in SW480 and HCT15 cells transfected with si-NC, si-TMPO-AS1, si-TMPO-AS1 + anti-miR-NC and si-TMPO-AS1 + anti-miR-143-3p via reverse transcription-quantitative PCR. Cell proliferation was measured in si-NC, si-TMPO-AS1, si-TMPO-AS1 + anti-miR-NC and si-TMPO-AS1 + anti-miR-143-3p groups in (B) SW480 and (C) HCT15 cells using a MTT assay. (D) Cell migration and invasion were detected in si-NC, si-TMPO-AS1, si-TMPO-AS1 + anti-miR-NC and si-TMPO-AS1 + anti-miR-143-3p groups in (E) SW480, (F) as well as in and (G) HCT15 cells using Transwell assays. (H) Western blotting results of cyclin D1 and p21 expression levels in (I) SW480 and (J) HCT15 cells transfected with si-NC, si-TMPO-AS1, si-TMPO-AS1 + anti-miR-NC and si-TMPO-AS1 + anti-miR-143-3p. (K) Western blotting results of MMP-2 and MMP-9 in (L) SW480 and (M) HCT15 cells transfected with si-NC, si-TMPO-AS1, si-TMPO-AS1 + anti-miR-NC and si-TMPO-AS1 + anti-miR-143-3p. *P<0.05 vs. si-NC; #P<0.05 vs. si-TMPO-AS1 + anti-miR-NC. miR, microRNA; si, short interfering; CRC, colorectal cancer; NC, negative control; MMP, matrix metalloproteinase; AS, antisense; OD, optical density.