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. 2020 Aug 27;13:432. doi: 10.1186/s13071-020-04266-0

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 8 — Maximum Likelihood (ML) mtDNA tree inferred from 17 nucleotide sequences. The analysed sequences included four mitochondrial genomes sequenced in this study (highlighted) and annotated reference genomes available on GenBank. The four mtDNA genomes analysed in this study were: R. microplus KF13 and KSF2, R. appendiculatus KF10 and R. decoloratus KBF6. Six R. microplus genomes and one genome each for R. annulatus, R. geigyi, R. sanguineus, R. turanicus and H. longicornis from GenBank were included. A partial mtDNA genome of R. appendiculatus (KC503257) and an unverified R. decoloratus genome (KY457525) available on GenBank were used to compare the genome sequences of R. appendiculatus and R. decoloratus. The tree was reconstructed using the General Time Reversible (GTR) (Nei & Kumar [32]) in MEGA X. There was a total of 18,435 positions in the final dataset