Table 1.

Details of primers used for mammalian DNA and B. bovis qPCR detection assays

Target gene	Primer/probe name	Sequence	Size (bp)	References
18S	Reverse: S0933_BoR_18S	AGTCGTGCGTCATCGACAAA	20	Kim et al. [26]
18S	Forward: S0934_BoF_18S	AGCAGGTTTCGCCTGTATAATG	22
18S	Probe (5′-FAM-3′): S0935_BoP	CCTTGTATGACCCTGTCGTACCGTTGG	27
Cytochrome b	Forward: S0936_bovisF160_Cytb	ATATGTTTGCATTTGCTG	18	Zhang et al. [27]
Cytochrome b	Reverse: S0937_bovisR249_cytb	CTCCAAACCAATATGAAAG	19
Cytochrome b	Probe (5′-HEX-3′): S0938_bovis_cytb	CAAACCATAAAGTCATCGGTATATCCTAC	29
GAPDH	Forward: S0631_Dog_F	TCAACGGATTTGGCCGTATTGG	22	Nijhof et al. [25]
GAPDH	Reverse: S0634_Dog_R	TGAAGGGGTCATTGATGGCG	20
GAPDH	Probe (5′-HEX-3′): S0632_Dog	CAGGGCTGCTTTTAACTCTGGCAAAGTGGA	30

Note: The fluorescence label of the Taqman probes is shown in brackets