Fig. 3.

Upregulating phospholipid synthesis or constraining precursor influx rescues endomembrane defects in TAG production defective cells. a, b Phospholipid production was upregulated by (1) knocking out OPI1 or (2) supplying key reactants (inositol, choline, and ethanolamine/ICE). Precursor influx was constrained by (1) 100-fold reduction of glucose supply (0.02% glucose), (2) chemical inhibition of fatty acid synthase (cerulenin), (3) elimination of major fatty acyl-CoA synthetases (faa1Δ faa4Δ), or (4) elimination of a key lysoPA acyltransferase (slc1Δ). a Representative images presented as in Fig. 1a. Scale bar, 2 μm. b Quantification of cells displaying organelle defects. Error bar, standard deviation, n = 3