Effects of blocking or stimulating the prostaglandin E2 receptor 1 on endoplasmic reticulum stress-related proteins. Images were captured with a Hitachi VKC150 camera. (A) Immunohistochemistry was used to visualize the expression of GRP78, TRPC1 and PERK (magnification, ×400). (B) Semi-quantitative analysis of GRP78, TRPC1 and PERK expression. *P<0.05. Con, control; WT, wild-type; GRP78, glucose-regulated protein 78; TRPC1, transient receptor potential channel 1; PERK, protein kinase R-like endoplasmic reticulum kinase; 17-pt-PGE2, 17-phenyl-trinor-prostaglandin E2 ethyl amide. Effects of blocking or stimulating the prostaglandin E2 receptor 1 on endoplasmic reticulum stress-related proteins. Images were captured with a Hitachi VKC150 camera. Western blotting was performed to detect GRP78, TRPC1 and PERK expression in groups treated with (C) SC-19220 and (D) 17-pt-PEG2 24 h following treatment with 10 ng/ml TGF-β1. *P<0.05. Con, control; WT, wild-type; GRP78, glucose-regulated protein 78; TRPC1, transient receptor potential channel 1; PERK, protein kinase R-like endoplasmic reticulum kinase; TGF-β1, transforming growth factor-β1; 17-pt-PGE2, 17-phenyl-trinor-prostaglandin E2 ethyl amide.