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. 2020 Jul 23;22(4):2887–2895. doi: 10.3892/mmr.2020.11353

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Copyright: © Chen et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — Effects of blocking or stimulating the prostaglandin E2 receptor 1 on the TGF-β1-induced upregulation of PGE2 and apoptosis of mouse MCs. (A) PEG2 concentration was detected in each group at 24 h after 10 ng/ml TGF-β1 treatment. (B) Apoptotic rates were measured in the MCs using fluorescence-activated cell sorting analysis after Annexin-V/PI staining. *P<0.05, **P<0.01. TGF-β1, transforming growth factor-β1; PGE2, prostaglandin E2; MCs, mesangial cells; PI, propidium iodide; WT, wild-type; 17-pt-PGE2, 17-phenyl-trinor-prostaglandin E2 ethyl amide.