Figure 4.

PRRX1 overexpression increases p-PI3K and p-AKT expression and decreases PTEN. Simple western blotting was used to detect the phosphorylation levels of (A) PI3K and (B) AKT. (C) The ratio of p-PI3K/PI3K and (D) p-AKT/AKT was calculated and presented. In addition, reverse transcription-quantitative PCR assays and simple western blotting were used to detect the (E) mRNA and (F and G) protein levels of PTEN. Values are expressed as mean ± standard deviation. **P<0.01 vs. BC and NC; ns, no significance; PRRX1, paired-related homeobox 1; BC, blank control; NC, negative control; ns, not significant.