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. 2020 Aug 3;22(4):3111–3116. doi: 10.3892/mmr.2020.11403

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Copyright: © Su et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — Effects of IFN-γ on the expression levels of Bcl-2, Bax, Bak, cleaved caspase-3, p-JAK1, p-JAK2 and p-STAT1. (A) Western blotting was used to analyze the expression levels of the proteins in human epidermal melanocytes following the treatment with 100, 200 or 400 ng/ml IFN-γ. (B) Quantification of the western blotting bands in part A. Bcl-2, Bax, Bak, cleaved caspase-3 were normalized to GAPDH. p-JAK1, p-JAK2 and p-STAT1 were normalized to JAK-1, JAK-2 or STAT1, respectively. *P<0.05 vs. control. p-, phosphorylated; IFN, interferon; JAK, janus kinase; Bak, Bcl-2 homologous antagonist killer.