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. 2020 Jul 15;22(4):2723–2732. doi: 10.3892/mmr.2020.11343

Figure 5.

Figure 5.

Impacts of miR-15b-5p and GDI2 on the proliferation and invasion of THCA cells. GDI2 expression was reduced or induced by si-GDI2 or pcDNA3.1-GDI2 in SW1736 and K1 cells both at (A) mRNA and (B) protein levels, (C) which were semi-quantified from western blot analysis. Control group cells were treated with a mixture of the scrambled siRNAs and pcDNA3.1. Proliferation of (D) SW1736 and (E) K1 cells were attenuated or accelerated by miR-15b-5p agomir or miR-15b-5p antagomir, which was restored by pcDNA3.1-GDI2 or si-GDI2. Inhibitory or stimulative effect of miR-15b-5p agomir or miR-15b-5p antagomir on (F) SW1736 and (G) K1 cell invasion was weakened by pcDNA3.1-GDI2 or si-GDI2. Scale bar, 200 µm. pcDNA3.1-GDI2 or si-GDI2 increased or decreased the expression levels of MMP2 and MMP9, which were caused by miR-15b-5p agomir or miR-15b-5p antagomir, in (H) SW1736 and (I) K1 cells. Control group cells were treated with the mixture of miR-15b-5p agomir control and pcDNA3.1 in D, F and H, or the mixture of miR-15b-5p antagomir control and the scrambled siRNAs in E, G and I. All experiments were repeated three times. *P<0.05, **P<0.01 vs. control group; ##P<0.01 vs. agomir group; &&P<0.01 vs. the antagomir group. miR, microRNA; GDI2, GDP dissociation inhibitor 2; siRNA, small interfering RNA; MMP, matrix metalloproteinase; CCK-8, Cell Counting Kit-8; OD, optical density.