Figure 6.

R.I flagellin may regulate miR-223-3p expression through TLRs but not TLR5. (A) RT-qPCR analysis was performed to determine mRNA TLR5 expression in THP-1 macrophages. (B) THP-1 macrophages were treated with LPS/ATP+R.I flagellin and transfected with si-TLR5. RT-qPCR analysis was performed to determine miR-223-3p expression. Data are presented as the mean ± standard error of the mean (n=3/group). *P<0.05. R.I, Roseburia intestinalis; miR, microRNA; TLR, Toll-like receptor; si, small interfering; NC, negative control; n.s, no significance.