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. 2020 Jul 23;22(4):2957–2965. doi: 10.3892/mmr.2020.11354

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Copyright: © Wang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — Mannose induces G0/G1 phase arrest in NSCLC cells in vitro. (A-D) Flow cytometry was performed to assess the effects of 30 mM mannose on the cell cycle distribution of NSCLC cells in vitro. (E and F) Western blotting was conducted to evaluate the effects of different concentrations of mannose (0, 30 and 60 mM) on the expression of PCNA and p21, regulators of cell cycle progression. Data are presented as the mean ± standard deviation. *P<0.05, **P<0.01, ***P<0.001. One-way ANOVA was used in parts (A) and (B). NSCLC, non-small cell lung cancer; ctrl, control; PCNA, proliferating cell nuclear antigen.