Figure 4.

Mannose reduces the migratory and invasive abilities of lung cancer cells in vitro. (A) Transwell migration assays were used to evaluate the effects of mannose on the migratory abilities of A549 and H1299 cells (scale bar =50 µm). (B) Transwell invasion assays were used to evaluate the effects of mannose on the invasive abilities of A549 and H1299 cells (scale bar =50 µm). (C and D) Western blotting was conducted to evaluate the effects of different concentrations of mannose (0, 30 and 60 mM) on the expression of epithelial-mesenchymal transition-associated proteins, including MMP2, E-cadherin and N-cadherin. Data are presented as the mean ± standard deviation. *P<0.05, **P<0.01, ***P<0.001. MMP2, matrix metalloproteinase 2; ctrl, control.