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. 2020 Aug 19;22(4):3482–3492. doi: 10.3892/mmr.2020.11438

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Copyright: © Xu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — SMA expression in developing placentas of PGRN^−/− and WT mice on E14.5 and E17.5. Immunofluorescence evaluation of SMA in (A) the placentas of PGRN^−/− mice on E14.5, (B) the placentas of WT mice on E14.5, (C) the placentas of PGRN^−/− mice on E17.5 and (D) the placentas of WT mice on E17.5. Western blot analysis was performed on placental tissue on (E) E14.5 and (F) E17.5. Scale bar, 50 µm. *P<0.05. SMA, smooth muscle actin; -/-, PGRN^−/− mice; PGRN, progranulin; WT, wild-type; E, embryonic day; TGC, trophoblast giant cells; fbv, fetal blood vessels; mrbc, maternal red blood cells; frbc, fetal red blood cells; ACTB, β-actin.