Figure 5.

eNOS expression in the developing placenta is lower in PGRN^−/− than in WT placentas on both E14.5 and E17.5, particularly in the labyrinthine layer of the placenta. Immunohistochemistry was performed on serial placental sections from (A) E14.5 and (B) E17.5 to determine eNOS expression. (A and B) Boxed areas in (a) are magnified in (c). Boxed areas in (b) are magnified in (d). (C) Western blotting and relative gray analysis was performed on placentas on E14.5 and E17.5. Scale bar, 500 µm; magnified scale bar, 50 µm. **P<0.01. eNOS, endothelial nitric oxide synthase; -/-, PGRN^−/− mice; PGRN, progranulin; WT, wild-type; E, embryonic day; MD, maternal decidua; JZ, junctional zone; LB, labyrinthine layer; ACTB, β-actin.