Figure 3.

Rheb is a direct target gene of miR-4654. (A) Sequence analysis of binding sites between miR-4654 and the WT Rheb 3′-UTR according to TargetScan. The Rheb 3′-UTR MUT sequence is also shown. (B) Dual-luciferase reporter assay was performed in 293T cells co-transfected with the plasmid containing WT or MUT Rheb 3′-UTRs and miR-4654 mimic or mimic-NC. Data are presented as the mean ± SD. N=3, ***P<0.001 vs. mimic-NC. 3′-UTR, 3′-untranslated region; miR, microRNA; WT, wild-type; MUT, mutant.