Figure 6.

miR-4654 promotes the formation of autophagosomes in HSFBs following resveratrol treatment. HSFBs were infected with adenoviruses expressing GFP-LC3 fusion protein to obtain a stable GFP-LC3 cell line. Different treatments/transfections were applied in the stable cell line, including resveratrol treatment alone, resveratrol + miR-4654 mimic transfection, resveratrol + miR-4654 mimic + Rheb OE transfection, resveratrol + miR-4654 inhibitor transfection + Rheb KD, resveratrol + miR-4654 inhibitor transfection and the control group. After 72 h, DAPI was used to stain cells and the cells were observed at a magnification of ×200 using confocal microscopy. Scale bar=20 µm. The GFP-LC3 fluorescence intensity was normalized against the control group and determined using Image J software. Data are presented as the mean ± SD, n=3, **P<0.01; ^##P<0.01 vs. the resveratrol group. LC3, microtubule-associated protein 1A/1B-light chain 3; OE, overexpression; KD, knockdown; Control, mimic-NC + inhibitor-NC + PBS + vector group; HSFBs, hypertrophic scar-derived fibroblasts; miR, microRNA.