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Primer sequences used to detect gene expression in human fibroblast-like synoviocytes by reverse transcription-quantitative PCR.
| Gene | Sequence (5′-3′) |
|---|---|
| TLR2 | F: AAGCACTGGCCAAAGTCTTG |
| R: TGTCCTGTGACATTCCGACA | |
| MYD88 | F: CCTGCAGAGCAAGGAATGTG |
| R: GTCGCAGACAGTGATGAACC | |
| GAPDH | F: GGGTGTGAACCATGAGAAGT |
| R: GACTGTGGTCATGAGTCCT |
TLR2, toll-like receptor 2; MYD88, myeloid differentiation factor 88; F, forward; R, reverse.
