TABLE 5.
Yeast strains used in this study.
| Strain | Genotype | References |
|---|---|---|
| BY4741 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 [rho+] | [43] |
| BY4741[rho0] | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 [rho0] | This study |
| BY4742 | MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0 [rho+] | [43] |
| Δmip1 | MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0 [rho0] | [27, 29] |
| J1361 | MATa CEN1-16:pGal-K.lactis-URA3 kar1Δ15 lys2Δ rad5-535 leu2-3,112 can1-100 his3-11,15 trp1-1 [rho+] | [60] |
| J1361 [rho0] | MATa CEN1-16:pGal-K.lactis-URA3 kar1Δ15 lys2Δ rad5-535 leu2-3,112 can1-100 his3-11,15 trp1-1 [rho0] | This study |
| J1361 [ARG8m rho+] | MATa CEN1-16:pGal-K.lactis-URA3 kar1Δ15 lys2Δ rad5-535 leu2-3,112 can1-100 his3-11,15 trp1-1 [ARG8m rho+] | This study |
| J1362 | MATα CEN1-16:pGal-K.lactis-URA3 kar1Δ15 lys2Δ rad5-535 leu2-3,112 can1-100 his3-11,15 trp1-1 [rho+] | [60] |
| JC8 [ARG8m rho+] | MATa kar1-1 leu1 [ARG8m rho+] | [50, 69, 87] |
| Y8205 | MATα can1Δ::STE2pr-Sp_his5 lyp1Δ::STE3pr-LEU2 his3Δ1 leu2Δ0 ura3Δ0 [rho+] | [68] |
| YDTL88 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 arg8Δ::HIS3MX6 [rho0] | This study |
| YKO strains1 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 orfΔ::kanMX4 [rho+] | [27, 29] and this study |
| YKO strains [rho0] | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 orfΔ::kanMX4 [rho0] | This study |
| YKO strains Δarg8 | MATα lyp1Δ::STE3pr-LEU2 his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 orfΔ::kanMX4 arg8Δ::natNT2 [rho+] | This study |
| YKO strains Δarg8 [rho0] | MATα lyp1Δ::STE3pr-LEU2 his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 orfΔ::kanMX4 arg8Δ::natNT2 [rho0] | This study |
| YKO strains Δarg8 [ARG8m rho+] | MATα lyp1Δ::STE3pr-LEU2 his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 orfΔ::kanMX4 arg8Δ::natNT2 [ARG8m rho+] | This study |
| heterozygous YKO strains Δarg8 [ARG8m rho+] | MATa/α LYP1/lyp1Δ::STE3pr-LEU2 his3Δ1/his3Δ1 leu2Δ0/leu2Δ0 met15Δ0/met15Δ0 ura3Δ0/ura3Δ0 ORF/orfΔ::kanMX4 arg8Δ::natNT2/arg8Δ::HIS3MX6 [ARG8m rho+] | This study |
| YMS001 | MATα CAN1 lyp1Δ::STE3pr-LEU2 his3Δ1 leu2Δ0 ura3Δ0 met15Δ0 [rho+] | This study |
| YMS002 | MATα CAN1 lyp1Δ::STE3pr-LEU2 his3Δ1 leu2Δ0 ura3Δ0 met15Δ0 arg8Δ::natNT2 [rho+] | This study |
| YMS003 | MATα CAN1 lyp1Δ::STE3pr-LEU2 his3Δ1 leu2Δ0 URA3 met15Δ0 arg8Δ::natNT2 [rho+] | This study |
| YMS004 | MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0 [pRS415 LEU2] [rho0] | This study |
| YMS005 | MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0 [pRS415 LEU2] [ARG8m rho+] | This study |
1
Yeast knock-out strains from the yeast deletion collection