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. 2020 Aug 4;22(4):3358–3366. doi: 10.3892/mmr.2020.11409

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Copyright: © Yu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — miR-33a-5p overexpression or HIF1α depletion suppresses HemEC proliferation, migration and invasion. (A-C) HemECs were transfected with miR-33a-5p mimics or miRNA NC. (A) The expression levels of miR-33a-5p were analyzed by RT-qPCR. (B) The relative proliferation was analyzed using a CCK-8 assay. (C) Migratory and (D) invasive abilities were analyzed by Transwell and Matrigel assays, respectively. (F-J) HemECs were transfected with HIF1α or NC siRNAs. (E) The expression levels of HIF1α were analyzed by RT-qPCR. (F and G) The protein expression levels of HIF1α were analyzed by western blotting. (H) The relative proliferation of HemECs was analyzed using a CCK-8 assay. (I) Migratory and (J) invasive abilities of HemECs were analyzed by Transwell and Matrigel assays, respectively. HemECs were transfected with miR-33a-5p mimics or miRNA NC and the expression levels of HIF1α (K) mRNA were analyzed by RT-qPCR and (L) protein was analyzed by western blotting. **P<0.01 and ***P<0.001 vs. NC group at the same time point or as indicated in figure. CCK-8, Cell Counting Kit-8; HemEC, hemangioma endothelial cells; HIF1α, hypoxia-inducible factor 1α; miR, microRNA; NC, negative control; RT-qPCR, reverse transcription-quantitative PCR; si, small interfering RNA.