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. 2020 Aug 4;22(4):3358–3366. doi: 10.3892/mmr.2020.11409

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Copyright: © Yu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 5. — NEAT1/miR-33a-5p/HIF1α axis regulates the NF-κB signaling pathway. The protein expression levels of p-NF-κB (p65) and NF-κB (p65) were analyzed by western blotting following transfection of HemEC cells with (A) NEAT1 siRNAs, (B) miR-33a-5p mimics or (C) HIF1α siRNA. The mRNA expression levels of c-myc were analyzed by reverse transcription-quantitative PCR following transfection of HemEC cells with (D) NEAT1 siRNAs, (E) miR-33a-5p mimics or (F) HIF1α siRNA. **P<0.01 and ***P<0.001. HIF1α, hypoxia-inducible factor 1α; miR, microRNA; NC, negative control; NEAT1, nuclear paraspeckle assembly transcript 1; si, small interfering RNA.