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Mouse genotype identification primers and probes for dual probe PCR.
| Name | Primer/probe | Sequence (5′-3′) | Product length (base pairs) |
|---|---|---|---|
| apoM+/+ mouse | Forward primer | CGGAAGTGGACATACCGATTG | 105 |
| Reverse primer | AAACGGCTAAGGGAGACGAGA | ||
| Probe | FAM-CTGAAGGTTGGTTCCCACCAGCCC-BHQ1 | ||
| apoM−/− mouse | Forward primer | GCTCCTGCCGAGAAAGTATCC | 98 |
| Reverse primer | CGATGTTTCGCTTGGTGGTC | ||
| Probe | CY5-TGCAATGCGGCGGCTGCATACGCT-BHQ2 |
