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. Author manuscript; available in PMC: 2020 Aug 28.
Published in final edited form as: Epilepsia. 2020 Apr 3;61(5):856–867. doi: 10.1111/epi.16490

Figure 5.

Figure 5

Granule layer-associated reelin (RLN)- and nitric oxide synthase (NOS)-immunoreactive interneurons. Ventral hippocampus in a control (A,B) and epileptic rat (C,D). m = molecular layer, g = granule cell layer, h = hilus. E Method to analyze coronal sections. Nissl stained sections from a control rat show the granule cell layer analyzed at different levels of the septotemporal axis of the hippocampus: red outline = septal (dorsal) dentate gyrus; green outline = temporal (ventral) dentate gyrus. E2,6 are the same section. E3,5 are the same section. E4 was the most posterior section that included granule cell layer. Results from the dorsal and ventral parts of the granule cell layer were plotted as section −1 and 1, respectively, in panels F,G. Granule cell layer-associated RLN- (F) and NOS-positive neuron profiles/section (G). Number of granule cell layer associated RLN- (H) and NOS-positive neurons (I) in the entire hippocampus (total), dorsal hippocampus, and ventral hippocampus. *p < 0.01, **p < 0.001, t test. For NOS, there also is a plot of the number in ventral hippocampi of epileptic rats without versus with a significant ictal onset site.