Fig. 4.

Simultaneous editing of adenine and cytosine by sgBE. a Architecture of sgABE. b Efficiency of adenine editing with sgABEs. HEK293T cells were transfected with plasmids expressing sgABEs targeting a set of three different sites. A-to-G editing efficiencies were analyzed by Sanger sequencing and EditR calculating. Each experiment was repeated at least three times. Data are represented as mean ± SEM. c Architectures showing different combinations of ABE+CBE. d Efficiency of cytosine or/and adenosine editing with different ABE+CBE combinations or single ABE/BEs. HEK293T cells were transfected with plasmids expressed indicated base editors or their combinations. C-to-T and A-to-G editing efficiencies were analyzed by Sanger sequencing and EditR calculating. Each experiment was repeated at least three times. Data are represented as mean ± SEM. e Efficiency of cytosine or/and adenosine editing with different ABE+CBE combinations and STEME systems. C-to-T and A-to-G editing efficiencies were analyzed by deep sequencing. Each experiment was repeated at least three times. Data are represented mean ± SEM; ^nsp > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001. f The ratio of single and simultaneous editing products generated by different ABE+CBE combinations and STEME systems at 4 target sites. Each experiment was repeated at least three times. Data were represented as mean ± SEM; ^nsp > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001