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. Author manuscript; available in PMC: 2020 Nov 13.
Published in final edited form as: Sci Transl Med. 2020 May 13;12(543):eaaz0802. doi: 10.1126/scitranslmed.aaz0802

Fig. 8. CRISPRi-mediated HIV-1–specific inhibition restores HIV-1–driven aberrant host gene transcription to that of uninfected cells.

Fig. 8.

From CRISPR-ready, gRNA transduced uninfected and HIV-1–infected Jurkat T cell clones 8B10 (in which HIV-1 is integrated into VAV1), 1G2 (in which HIV-1 is integrated into RAP1B), and 1D7 (in which HIV-1 is integrated into SPECC1); HIV-1–green fluorescent protein–positive (GFP+) cells in CRISPRa/HIV-1 gRNA and CRISPRa/nontargeting gRNA systems were sorted for RNA landscape analysis at the integration site. HIV-1–GFP− cells in CRISPRi/HIV-1 gRNA and CRISPRi/nontargeting gRNA systems were sorted similarly. Peaks show normalized RNA transcription in the corresponding genes VAV1 (A), RAP1B (B), and SPECC1 (C).