Figure 1. Pole^P286R is a fully penetrant monoallelic driver of endometrial cancer and cooperates with Msh2 deficiency.

(A) Diagram showing single-generation breeding scheme for conditional Pole^P286R/+ endometrial knockin mice. Genotypes shown are germline (determined by tail PCR). (B) Gross photographs of uteri. Images placed on white background. Scale bar: 1 cm. (C) Survival analyses, P value per log-rank test. (D) Uterus weight at necropsy as metric for uterine tumor burden; n = 7 per group, P value per 2-tailed Mann-Whitney U test. (E) Histologic features of uterine primary cancers, H&E-stained sections. Top panels show architecturally well-differentiated endometrioid adenocarcinomas with atypical mitoses (arrows). Middle panels show more poorly differentiated adenocarcinomas without gland formation. Bottom panels show cases with squamous differentiation (sq) or striking nuclear atypia (right panel) as described for human POLE^P286R cancers. Scale bars: 50 μm.