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. 2020 Jul 23;5(14):e134888. doi: 10.1172/jci.insight.134888

Figure 4. Anillin is a direct target of miR-96 and miR-183.

Figure 4

(A) Prediction of anillin (ANLN) as a target gene of miR-96 and miR-183 by TargetScan. (B) Luciferase assay for ANLN 3′-UTR (n = 3/group). Luciferase activity at 48 hours after cotransfection of HEK293T cells with both miR-96 and miR-183 or control mimics and ANLN 3′-UTR or ANLN 3′-UTR mutated (mut). Relative gene expression of Anln after miR-96 and miR-183 overexpression (C) or inhibition (D) in mouse cardiac endothelial cells (MCECs). (n = 4/group). **P < 0.01 vs. control mimic or anti-miR (Student’s t test). Data are shown as mean ± SEM.