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. 2020 Jul 23;5(14):e129070. doi: 10.1172/jci.insight.129070

Figure 8. Effect of ELA peptides on APLNR recycling.

Figure 8

(A) HEK293 cells expressing APLNR-EGFP were analyzed under confocal microscopy after activation with ELA peptides (100 nM) at indicated time periods. Scale bar: 20 μm. White arrows indicate internalized receptors and red arrows indicate cell surface receptors. (B) HEK-APLNR cells were treated with ELA peptides and analyzed 30 minutes, 60 minutes, or 120 minutes after washout of the peptides. Scale bar: 50 μm. All experiments were repeated 3 times.